Part:BBa_I716209:Design

ceaB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716209

sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc

Source

PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716209

sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc